Figure 5.

Accumulation of LOX and icl transcripts during in vitro senescence of cucumber cotyledons. Cotyledons were excised from cucumber seedlings grown for 10 d under a 14-h light/10-h dark photoperiod and then kept in the dark for the number of days indicated. Total RNA (20 μg) isolated from the cotyledons was loaded in each lane, separated with a denaturing gel, transferred to a nylon membrane, and then sequentially probed with the lox-1 3′ probe (A), the lox-1 internal probe (B), and the icl probe (C).