Figure 6.

Identity between amino acid sequences determined with soluble LOX and lipid body LOX purified from cucumber cotyledons. The amino acid sequence deduced from the lox-1 cDNA sequence (accession no. U25058) is shown. The sequences determined with peptides derived from soluble LOX are underlined; those from lipid body LOX are double underlined. Three differences can be found between lox-1 and cslblox (accession no. X92890) at the nucleotide level. Two of the three differences produce one amino acid substitution (Ser to Ala), as shown at position 491, although the amino acid sequence around Ser-491, namely IELUSLPHP, is highly conserved within plant LOXs and no such substitution can be found. The other difference is silent, and we do not know whether the difference is caused by a sequencing artifact or by the different varieties used as the gene source.