Figure 4.

Selective sequestration of FcεRI⁺ neutrophils in the brain of mice infected with PbANKA. C57BL/6 mice were infected with the ECM-inducing PbANKA (top) or with the ECM noninducing PbNK65 (bottom). At day 6 and 20 after infection, respectively, they were perfused and brains were collected. Isolated cells were labeled with anti–CD45-PerCP, 7/4-FITC, Ly6G-PE, and FcεRI-APC. (a and h) Cells gated on CD45⁺-7/4⁺. (b and i) 7/4⁺-Ly6G⁺ neutrophils. A population of neutrophils expressing FcεRI is shown in c and d, as compared to cells labeled with an isotype control antibody (e). A subpopulation of 7/4⁺-Ly6G⁺ cells was also positive for FcεRI (f). Similar analysis for mice infected with the PbNK65 parasite strain was performed (j, k, l, and m). Quantitative analysis of Ly6G⁺-7/4⁺-FcεRI⁺ neutrophil populations was performed on five individual PbANKA-infected mice (g) and on PbNK65-infected mice (n). ** indicates significant differences as compared with control IgG (n = 5; P < 0.007). Results were obtained from two independent experiments.