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. 1999 Apr;119(4):1297–1304. doi: 10.1104/pp.119.4.1297

Figure 5.

Figure 5

A, Theoretical restriction fragment lengths of PCR product from the genomic DNA or cDNA. The structure of Md-ACS1 is indicated as described in the legend of Figure 1. The locations of the primers used in the PCR are shown by arrows. H, HindIII site; (X), ACS1-2-specific XhoI site. B, Fragment lengths of HindIII/XhoI double-digested PCR products. Lane M, φX174/HaeIII; lane 1, genomic DNA of cv Golden Delicious; lane 2, RT-PCR products from climacteric cv Golden Delicious fruit; lane 3, RT-PCR product from climacteric cv Fuji fruit.