Figure 1.

Subcellular localization of palmitoyl-CoA-dependent ACAD activity in embryonic axes from early-germinating sunflower seeds. Crude mitochondria from embryonic axes of early-germinating sunflower seeds were further separated by isopyknic centrifugation on a 20% Percoll gradient. Fractions of 2 mL were collected and assayed for the activity of mitochondrial fumarase (A), palmitoyl-CoA-dependent ACAD (B), peroxisomal ACOX (C), and peroxisomal catalase (D). The scale of volumes from 0% to 100% ranges from the top to the bottom of the Percoll gradient. Separation of organelles and enzyme activity measurements were carried out as described in Methods.