Table IV.
Total palmitoyl-CoA-dependent ACAD activity in crude protein extracts from maize and sunflower tissues
| Tissue | Enzyme Activity
|
|
|---|---|---|
| ACAD | Acyl-CoA thioesterase | |
| nmol min−1 g−1 fresh wt | ||
| Maize plantlets | ||
| Whole seminal root | 4 ± 1 | 12 ± 2 |
| Carbohydrate-starved root tip | 32 ± 8 | 100 ± 10 |
| Early-germinating sunflower seeds | ||
| Embryonic axes | 30 ± 10 | NDa |
| Cotyledons | 25 ± 5 | 100 ± 10 |
ACAD and acyl-CoA thioesterase activities were measured as described in Methods in the presence of 50 μm palmitoyl-CoA. Maize and sunflower tissues were obtained and protein extraction was carried out as described in Methods. Results are the means ± range or ± se from two or three experiments.
ND, Not detected.