Table V.

Chain-length substrate specificity of ACAD activities in partially purified ACAD preparations from embryonic axes of early germinating sunflower seeds

Substrate	ACAD1	ACAD2
50 μm	%
Butyryl-CoA	NDa	78
Hexanoyl-CoA	39	59
Octanoyl-CoA	63	75
Decanoyl-CoA	100	98
Myristoyl-CoA	84	100
Palmitoyl-CoA	100	16
Stearoyl-CoA	62	ND

ACAD was purified as described in Methods. Partially purified ACAD1 and ACAD2 preparations were obtained as described in the legend for Figure 2. ACAD activities in ACAD1 and ACAD2, which were measured as described in “Materials and Methods,” are given as activities relative to palmitoyl-CoA-dependent (20 nmol min⁻¹ mg⁻¹) and myristoyl-CoA-dependent (850 nmol min⁻¹ mg⁻¹) specific activities, respectively.

^aND, Not detected.