FIG. 4.

Effect of PEITC and SNAP on the expression of representative protein components of the mitochondrial respiratory complexes. (A) HL-60 cells were treated with 10 μM PEITC with or without pretreatment with NAC or catalase for 1, 3, and 6 h, analyzed on a 12% SDS-PAGE, and then probed for representative components of the mitochondrial complexes using the respective specific antibody cocktail. The arrows indicate a decrease in complex I component. (B) Raji cells were treated with 10 μM PEITC with or without pretreatment with NAC or catalase for 1, 3, and 6 h, and the representative components of mitochondrial respiration complexes were analyzed by western blotting. (C) HL-60 cells were treated with 4 mM SNAP for 1, 3, and 6 h or 5 μM PEITC for 3 h. Cell lysates were separated by 12% SDS-PAGE and blotted with a cocktail of antibodies against representative mitochondrial complex components. The arrow indicates a decrease in complex I component. (D) HL-60 cells were treated with 10 μM PEITC with/without NAC pretreatment, and cell lysates were separated by 12% SDS-PAGE and blotted with various redox-related proteins by using specific antibodies as indicated. SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.