FIG. 5.

Decrease of mitochondrial respiratory complex I subunit NDUFS3 induced by PEITC. (A) HL-60 cells were treated with 10 μM PEITC for 1 and 3 h in the presence or absence of pretreatment with NAC. Mitochondria were isolated, and complex I proteins were immunoprecipitated. The precipitated products were separated by 12% SDS-PAGE and stained with a sliver staining kit. The protein band near 26 kD was cut out for mass spectrometry analysis. (B) Mass spectrometry analysis showing the amino acid sequence of the protein fragment (VVAEPVELAQEFR), which matched to NDUFS3. (C) HL-60 and Raji cells were treated with 10 μM PEITC for 3 h, and the cell lysates were separated by 12% SDS-PAGE and blotted with a NDUFS3 monoclonal antibody. NDUFS3, NADH dehydrogenase Fe-S protein-3.