FIG. 6.

Effect of PEITC on cellular and mitochondrial GSH levels and ROS and membrane potential. (A) HL-60 cells were treated with 10 μM PEITC for various time points up to 12 h, and cellular GSH contents were determined. Each bar indicates the mean±SD from three experiments. (B) HL-60 cells were treated with 10 μM PEITC for 0.5, 1, and 3 h or 100 nM rotenone for 3 h. Mitochondria were then isolated, and the mitochondrial GSH contents were determined. Each bar indicates the mean±SD from three experiments. (C) Mitochondria were isolated from HL-60 cells, re-suspended in buffer, and then incubated with 10 μM PEITC for 0.5, 1, and 3 h or with 100 nM rotenone for 3 h. Mitochondrial GSH contents were determined. Each bar indicates the mean±SD from three experiments. *p<0.01; **p<0.001 by comparing to control. (D) HL-60 cells were treated with 10 μM PEITC for 0.5, 1, and 3 h; mitochondrial transmembrane potential and ROS levels were measured by flow cytometry by using rhodamine-123 and MitoSOX dye, respectively. (E) HL-60 cells were treated with 100 nM rotenone for 3 h, and mitochondrial membrane potential and ROS level were determined by flow cytometry by using rhodamine-123 and MitoSOX dye, respectively. GSH, glutathione.