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. 2011 Dec 15;15(12):2911–2921. doi: 10.1089/ars.2011.4170

FIG. 7.

FIG. 7.

Effect of BSO on cellular and mitochondrial GSH levels and rotenone-induced cytotoxicity. (A) HL-60 cells were treated with 500 μM BSO for various time points for up to 48 h, and cellular and mitochondrial GSH contents were determined. Each data point indicates the mean±SD from three experiments. (B) Raji cells were treated with 500 μM BSO for various time points up to 48 h, and cellular and mitochondrial GSH contents were determined. Each data point indicates the mean±SD from three experiments. (C) HL-60 cells were pretreated with BSO (500 μM) for 0 and 24 h, then each sample was incubated with rotenone (100 nM) in the presence of BSO for an additional 24 h. Cell viability was determined by annexin-V/PI double staining by using flow cytometry analysis. The number in each panel indicated % of death in cells. BSO, L-buthionine sulfoximine; PI, propidium iodide.