Figure 5.

Immunoblot analysis of tomato total mitochondrial proteins from fruits at different stages of ripeness (G, green; Y, yellow; O, orange; and R, red). Proteins were electrophoresed, transferred to nitrocellulose, and reacted with monoclonal antibodies against the S. guttatum AOX (left) or with polyclonal antibodies against the potato PUMP (right), as described in Methods. The total mitochondrial protein load was 150 μg for AOX detection and 30 μg for PUMP detection. The molecular mass markers appear on the left.