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. Author manuscript; available in PMC: 2012 Jul 23.
Published in final edited form as: Cytoskeleton (Hoboken). 2011 Aug 23;68(8):459–469. doi: 10.1002/cm.20524

Figure 8.

Figure 8

Eyespot positioning in cold-treated wild-type and pey1 cells. A: Immunofluorescence micrographs of a wild-type cell treated for 90 minutes at 0°C to depolymerize microtubules. Eyespot structure remains intact and retains approximately equatorial position as indicated by EYE3 and ChR1 staining (arrow in merge). B: Immunofluorescence micrographs of a pey1 cell showing incompletely depolymerized rootlet (arrowhead) while the eyespot retains its position. C: Immunofluorescence micrographs of a wild-type cell after 90 minutes at 0°C showing D4 rootlet remaining after depolymerization of other microtubule rootlets (arrow). Bars, 5 μm.