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. Author manuscript; available in PMC: 2011 Oct 25.
Published in final edited form as: Metabolism. 2009 Jun;58(6):835–842. doi: 10.1016/j.metabol.2009.03.002

Fig. 4.

Fig. 4

The effects of food restriction on STAT1, STAT3, ERK1/2, and p38 abundance and phosphorylation in rat liver at basal conditions and in response to IL-6 administration. Three groups were given a standard rodent chow diet for 14 days at ad libitum (control, n = 8), 25% reduction of ad libitum intake (25%, n = 8), or 50% reduction of ad libitum intake (50%, n = 8). From each group, 4 rats received saline injection (−IL-6); and another 4 rats received 20 µg/kg of IL-6 injection (+IL-6). After 5 minutes of injection, the liver was harvested. Liver extracts were directly blotted with anti- or antiphospho-STAT1, STAT3, ERK1/2, and p38 antibodies. Each lane in blots corresponds to an individual representative animal in the indicated group. The mean changes from 4 rats in arbitrary densitometry units normalized against an internal standard were compared. No significant differences were observed in different groups (ad libitum, 25%, and 50%) and different treatments (−IL-6 and +IL-6).