Figure 5.

In both d-7 (○) and d-12 (□) cells, the production of IP₃ increased with the osmostimulus. IP₃ levels were quantified in G. sulphuraria cultures stimulated for 90 s with increasing concentrations of methyl-Man (5, 10, 50, and 100 mm final concentration). Data are the averages of four values from two experiments.