Table 2.
Sequences of primers used in the study
| Name | Position of the primer and direction in HBV genome (bold characters) | Sequence (5′–3′) |
|---|---|---|
| F1 | 1703, Fw | YATAAGAGGACTCTTGGACT |
| R1 | 1018, Rv | GCAAANCCCAAAAGACCCA |
| HindPcPol-F2 | 1792, Fw | TACAAGCTTATTGGTCTGCGCACCAGC |
| HindPcPol-R2 | 764, Rv | TACAAGCTTCTTGGCCCCCAATACCAC |
| A3 | 2489, Rv | CCCMGTAAARTTYCCCACCTTAT |
| B3 | 245, Fw | GTCTAGACTCGTGGTGGACTTCTCTC |
| C3 | 550, Fw | ATGTWTCCCTCWTGTTGCT |
| D3 | 1864, Fw | TCAAGCCTCCAAGCTGTGCC |
| E3 | 2267, Fw | GGAGTGTGGATTCGCACT |
| A4 | 2270, Fw | GTGTGGATTCGCACTCCTCCAG |
| B4 | 1927, Rv | AAATTCTTTATAAGGGTCAATGT |
| C4 | 2817, Fw | TCACCATATTCTTGGGAACA |
| D4 | 57, Fw | TGCTGGTGGCTCCAGTTC |
| UDPS-Primer A | 703, Fw | GCCTCCCTCGCGCCATCAGGTAGGGCTTTCCCCCACTG |
| UDPS-Primer B | 1931, Rv | GCCTTGCCAGCCCGCTCAGCTCCAAATTCTTTATAWGGGTCRA |
| Fw T1855C | 1834, Fw | CTAATCATCTCTTGTACATGcCCCRCTGTTCAAGCCTCCAAGCTG |
| Rv T1855C | 1879, Rv | CAGCTTGGAGGCTTGAACAGYGGGgCATGTACAAGAGATGATTAG |
| C3-Rv | 569, Rv | AGCAACAWGAGGGAWACAT |
| pTriEx-Fw | – | TCTGCGCTCTGCTGAAGCCAGTTA |
| pTriEx-Rv | – | ACGATCGGAGGACCGAAGGAGCT |
The position and direction of the 5′ primer in the HBV genome (bold characters in the sequence), and the mutagenized position (bold lower cases) are indicated