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. 2011 Jul 15;39(19):8392–8403. doi: 10.1093/nar/gkr458

Figure 1.

Figure 1.

PXR represses the SULT1E1 gene in human primary hepatocytes and Huh7 cells. (A) Human primary hepatocytes were treated with RIF for 24 h in William's E medium, from which total RNAs were prepared and subjected to real-time PCR. The levels of the SULT1E1, STS and CYP3A4 mRNAs are expressed by taking those in the DMSO-treated cells as one. *P < 0.05 (DMSO versus RIF). (B and C) After 30 h infection with adeno-β-gal or adeno-hPXR, cells were treated with RIF for indicated time in FBS-free MEM (B) or were pre-treated with CHX (10 µg/ml) for 2 h, and then co-treated with RIF for another 16 h (C). Total RNAs were prepared at the indicated time points and subjected to real-time PCR. The levels of the SULT1E1, STS and CYP3A4 mRNAs are expressed taking those in the DMSO-treated Huh7 cells infected with adeno-β-gal as one. Bars represent the mean ± SD. *P < 0.05 (PXR + DMSO versus PXR + RIF).