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Religation assay for WT gyrase (A) and its mutant L518A (B). An amount of 100 ng of negatively supercoiled pBR322 was added in all reactions. An amount of 11 and 100 nM enzyme were added for WT and its mutant L518A (GyrB in the presence of equal molar GyrA), respectively. DNA alone is a negative control. Reaction times (in minutes) are indicated. R, relaxed pBR322; L, linear pBR322; S, supercoiled pBR322.
