Table 1.

Relative topoisomerase activities

Reactions	Peak I (%)	Peak II (%)	Peak III (%)	W661C	T546C	A543C
Supercoiling	13 ± 4	42 ± 8	100	<3 (% WT)	6 ± 1 (%WT)	<3 (% WT)
Decatenation	11 ± 2	55 ± 7	100	NT	NT	NT
Relaxationa	32 ± 8	70 ± 10	100	70 ± 10 (% WT)	25 ± 4(% WT)	20 ± 5(% WT)

^aRelaxation of negatively supercoiled pBR322.

All the activities are in the presence of wild-type GyrA. The topoisomerase activities of peaks I and II of GyrB are given as a percentage of the peak III; the topoisomerase activities of W661C, T546C and A543C mutants are given as a percentage of the wild-type enzyme. Data in table 1 are averaged from three separate experiments (mean ± standard deviations). NT, not tested.