Figure 3.

TPE4A is encoded by a single-copy gene. A, Genomic Southern-blot analysis showing that TPE4A is encoded by a single gene. Ten micrograms of pea genomic DNA was digested with EcoRI (E), XbaI (X), HindIII (H), and BamHI (B), and double digestions with combinations of these enzymes were also performed. The HindIII fragment of the cDNA clone was used as the probe. B, Deduced genomic organization of the TPE4A gene. Thick black lines represent the interrupted open reading frame, and thin lines represent the putative intron and the flanking regions of the gene. For simplicity, a single intron bearing the EcoRI site is shown.