Table 2.
Oxysterols and total cholesterol in control (C) and glycolaldehyde-treated macrophages (GAD)
| Macrophage | C | GAD | ||||
|---|---|---|---|---|---|---|
| - | HDL |
HDL + T0901317 |
- | HDL |
HDL + T0901317 |
|
| 7-ketocholesterol | 86.7 ± 16 | 72.8 ± 14* | 68.4 ± 14* | 83.8 ± 19 | 74.2 ± 13 | 79.8 ± 19 |
| 7β-hydroxicholesterol | 26.7 ± 9 | 20.4 ± 6 | 21.5 ± 10 | 25.5 ± 10 | 23.6 ± 6 | 22.8 ± 10 |
| 7α-hydroxicholesterol | 9.2 ± 4 | 8.1 ± 3 | 8.1 ± 5 | 9.4 ± 3 | 8.8 ± 2 | 8.6 ± 4 |
| cholesterol-5,6 β | 22.6 ± 9 | 20 ± 5 | 16.9 ± 6 | 24.2 ± 6 | 22.9 ± 6 | 20.5 ± 6 |
| cholesterol- 5,6 α | 7.1 ± 2 | 6.6 ± 2 | 5.5 ± 2 | 7.4 ± 2 | 6.2 ± 2 | 6.7 ± 3 |
| 24.25 epoxycholesterol | 3.8 ± 2 | 3.5 ± 2 | 2.8 ± 1 | 3.9 ± 2 | 3.7 ± 2 | 3.5 ± 2 |
| 27-hydroxycholesterol | 0.48 ± 0.2 | 0.51 ± 0.3 | 0.44 ± 0.2 | 0.55 ± 0.3 | 0.52 ± 0.2 | 0.48 ± 0.2 |
| 25-hydroxycholesterol | 0.35 ± 0.3 | 0.38 ± 0.1 | 0.42 ± 0.2 | 0.43 ± 0.2 | 0.37 ± 0.1 | 0.40 ± 0.2 |
| cholestan-3β,5α,6β-triol | 10 ± 3 | 8.3 ± 2 | 8.5 ± 5 | 10.5 ± 4 | 9.4 ± 3 | 9.2 ± 3 |
| total cholesterol | 244 ± 57 | 203 ± 47* | 191 ± 44* | 227 ± 52 | 214 ± 53 | 224 ± 50 |
Values were corrected by 5-alpha cholestane (internal standard) and are expressed as ng/μg of cell protein ± DP (n = 12).
* p < 0.05 comparison were made between cells maintained in the presence of culture medium alone with HDL or HDL + T0901317. One way ANOVA and Bonferroni post test (mean ± SD).