Table 4. Most abundant proteins based on the total spectra identified in ESP from adult H. polygyrus.
Protein Name | Blast2GO Seq. Description | Accession Numbers | P (%) | SC | UP | Closest Blastx Hit Description | Closest Blast HitSpecie | Closest Blast Hit Accession Number | Blastx Hit eValue |
L_272_T_4/4_C_0.700_3 | venom-allergen-like protein family member (vap-1) | L_272_T_4_0.700_3 | 100 | 132 | 19 | two-domain activation associated secreted protein ASP4 precursor | Ostertagia ostertagi | CAO00417.1 | 2E-89 |
L_566_T_2/6_C_0.476_3 | globin | L_566_T_2_0.476_3 L_566_T_3_0.571_3 L_566_T_4_0.524_3 L_566_T_5_0.762_2 L_566_T_6_0.762_3 | 100 | 82 | 5 | Globin-like host-protective antigen | Trichostrongylus colubriformis | P27613.1 | 3E-30 |
L_120_T_1/8_C_0.410_1 | secreted protein asp-2 | L_120_T_1_0.410_1 L_120_T_5_0.436_1 L_120_T_8_0.462_1 | 100 | 74 | 6 | secreted protein 4 precursor | Ancylostoma caninum | AAO63576.1 | 3E-26 |
L_384_T_8/9_C_0.306_5 | globin | L_384_T_8_0.306_5 | 100 | 73 | 10 | Globin-like host-protective antigen | Trichostrongylus colubriformis | P27613.1 | 3E-55 |
L_2405_T_4/4_C_0.700_4 | secreted protein asp-2 | L_2405_T_4_0.700_4 | 100 | 53 | 11 | secreted protein 6 precursor | Ancylostoma caninum | AAO63578.1 | 8E-20 |
L_2261_T_1/1_C_1.000_4 | globin | L_2261_T_1_1.000_4 | 100 | 47 | 7 | Globin-like host-protective antigen | Trichostrongylus colubriformis | P27613.1 | 4E-39 |
L_73_T_5/5_C_0.619_4 | vit-2 | L_73_T_5_0.619_4 | 100 | 46 | 10 | C. briggsae CBR-VIT-6 protein | Caenorhabditis briggsae | XP_002634040.1 | 6E-37 |
L_94_T_3/4_C_0.429_4 | C. elegans protein confirmed by T evidence | L_94_T_3_0.429_4 L_94_T_4_0.429_1 | 100 | 46 | 8 | LYSozyme family member (lys-8) | Caenorhabditis elegans | NP_495083.1 | 5E-30 |
L_122_T_10/10_C_0.312_3 | vitellogenin structural genes (yolk protein genes) family member (vit-2) | L_122_T_10_0.312_3 | 100 | 45 | 10 | VITellogenin structural genes (yolk protein genes) family member (vit-1) | Caenorhabditis elegans | NP_509305.1 | 1E-45 |
L_2367_T_1/3_C_0.375_1 | ll20 15kda ladder antigen | L_2367_T_1_0.375_1 | 100 | 44 | 17 | DVA-1 polyprotein | Dictyocaulus viviparus | Q24702.1 | 2E-145 |
L_93_T_5/6_C_0.444_2 | vitellogenin structural genes (yolk protein genes) family member (vit-2) | L_93_T_5_0.444_2 | 100 | 43 | 10 | VITellogenin structural genes (yolk protein genes) family member (vit-2) | Caenorhabditis elegans | NP_001123117.1 | 4E-53 |
L_207_T_17/20_C_0.196_1 | briggsae cbr-vit-5 protein | L_207_T_17_0.196_1 | 100 | 41 | 7 | VITellogenin structural genes (yolk protein genes) family member (vit-4) | Caenorhabditis elegans | NP_508612.1 | 3E-35 |
L_290_T_3/3_C_0.833_3 | briggsae cbr-vit-2 protein | L_290_T_3_0.833_3 | 100 | 35 | 4 | C. briggsae CBR-VIT-2 protein | Caenorhabditis briggsae | XP_002644638.1 | 4E-32 |
L_211_T_5/5_C_0.765_5 | vitellogenin structural genes (yolk protein genes) family member (vit-6) | L_211_T_5_0.765_5 | 100 | 30 | 7 | Vitellogenin-6 | Oscheius sp. (strain CEW1) | Q94637.1 | 8E-39 |
L_3_T_3/3_C_0.875_4 | vitellogenin structural genes (yolk protein genes) family member (vit-2) | L_3_T_3_0.875_4 | 100 | 28 | 7 | Vitellogenin-6 | Oscheius sp. (strain CEW1) | Q94637.1 | 6E-31 |
L_2733_T_1/1_C_1.000_3 | globin | L_2733_T_1_1.000_3 | 100 | 27 | 7 | Globin-like host-protective antigen | Trichostrongylus colubriformis | P27613.1 | 5E-56 |
L_6303_T_1/1_C_1.000_1 | ancylostoma-secreted protein 1 precursor | L_6303_T_1_1.000_1 | 100 | 27 | 2 | ancylostoma-secreted protein 1 precursor | Ancylostoma duodenale | AAD13339.1 | 2E-04 |
L_927_T_1/2_C_1.000_6 | NA | L_927_T_1_1.000_6 L_927_T_2_1.000_4 | 100 | 25 | 2 | ||||
L_160_T_5/5_C_0.733_4 | secreted protein asp-2 | L_160_T_5_0.733_4 | 100 | 23 | 7 | secreted protein 5 precursor | Ancylostoma caninum | AAO63577.1 | 3E-38 |
L_407_T_1/2_C_1.000_5 | vitellogenin structural genes (yolk protein genes) family member (vit-6) | L_407_T_1_1.000_5 | 100 | 23 | 3 | Vitellogenin-6 | Oscheius sp. (strain CEW1) | Q94637.1 | 4E-15 |
L_7106_T_1/1_C_1.000_2 | acetylcholinesterase 2 | L_7106_T_1_1.000_2 | 100 | 23 | 14 | putative neuromuscular acetylcholinesterase | Dictyocaulus viviparus | AAS49411.1 | 6E-169 |
Protein name identifiers are derived from the original transcriptome assembly nomenclature (L = locus, T = transcript, C = confidence) from which the conceptual translation frame number was added to the identifiers. P (%) = Protein identification probability (%); SC = Number of spectral counts; UP = Number of unique peptides.