Table 1.
Location of 23 microsatellites in the three-spined stickleback genome and primer sequences for nine-spined sticklebacks
| Locus | Three-spined stickleback genome | Microsatellite marker for nine-spined stickleback | ||||
|---|---|---|---|---|---|---|
| LG | Position (bp) | Repeat motif | Primer sequence (5'-3') | A | Allele size (bp) | |
| Ppsm1 | XII | 188976 | (CCT)6 | F: GGCTCTTCCGATGAGTTCTC | 6 | 298-308 |
| R: GTCTGCGCGTCAGCATCC | ||||||
| Ppsm2 | XII | 3625645 | (AC)24 | F: GCCTCCCAGTCCTCTGT | 5 | 175-183 |
| R: GCCATGGAGTACGACATCATG | ||||||
| Ppsm3 | XII | 4802750 | (GT)16 | F: GAACGATGATTAATTTCACTC | 42 | 161-271 |
| R: CTGACCCTGACTGGAGG | ||||||
| Ppsm4 | XII | 4843632 | (CA)15 | F: CCAGCTGCTCTGTTTTGTTAAC | 16 | 223-267 |
| R: CCTGGCCTCATTACAGTAAC | ||||||
| Ppsm5 | XII | 5616109 | (GAG)7 | F: ATCACGACTCTGAGGAGAG | 10 | 208-241 |
| R: TTCTTCAGCTCCACGGG | ||||||
| Ppsm6 | XII | 7178391 | (AC)12 | F: ATCGCCCTGCTGGTGGAG | 5 | 231-239 |
| R: GGAGCGCTGTTTCCGCC | ||||||
| Ppsm7 | XII | 7695994 | (GT)13 | F: GCAGCACTGTTGTCCAA | 9 | 73-143 |
| R: CTACTTGAACGATGCTC | ||||||
| Ppsm8 | XII | 9015356 | (GT)15 | F: CCAAAGGCAATTTCAAATCTC | 4 | 203-217 |
| R: GAATGACAGGCTGTTTGTCTG | ||||||
| Ppsm9 | XII | 10780938 | (TG)16 | F: CAAGATGGACTACTCAAGG | 3 | 247-252 |
| R: CTATCAACCTCTCCAGCTTC | ||||||
| Ppsm10 | XII | 12581231 | (TG)7 | F: GCTTAGTGTTAATTGGTTCCTG | 7 | 218-232 |
| R: TAGACCCTGAGGGTGTG | ||||||
| Ppsm11 | XII | 15639505 | (AT)8 | F: AACAACGGTGCTATCTCCTCT | 7 | 186-198 |
| R: TGGAATCCCATGCAGCGCAC | ||||||
| Ppsm12 | XII | 16027500 | (TG)8 | F: GCATGGTCATCATCTGGAG | 4 | 251-263 |
| R: ATGACACATGCATGGAGT | ||||||
| Ppsm13 | XII | 16138995 | (TG)13 | F: GCCTGCTACAAAGCTGA | 3 | 256-280 |
| R: CTTGAAGGACTCAAAGAAGCC | ||||||
| Ppsm14 | XII | 18276705 | (CA)11 | F: CCGATGGCCTGGTTCAC | 47 | 263-445 |
| R: GGCGTCATCATCACCGG | ||||||
| Pprm1 | III | 4656850 | (TG)15 | F: TGTGCTGCAGACCTCCAC | 5 | 202-214 |
| R: TGGCGTCACGGAGCTGAAG | ||||||
| Pprm2 | IV | 62968 | (CA)13 | F: GATACAGCTCCTGCTCCAG | 4 | 163-169 |
| R: CCAGGATGAACCAGGTGAG | ||||||
| Pprm3 | IV | 15800213 | (GT)21 | F: GGCTTCTATTTCTGCCTCCC | 23 | 344-392 |
| R: TACCTGAGCAGCTCGCAG | ||||||
| Pprm4 | V | 4991466 | (TGT)6 | F: GCTGGGCAGTATTCTGTGG | 7 | 288-306 |
| R: AACATCCTCATCCACAGCAGC | ||||||
| Pprm5 | XV | 21776 | (AC)16 | F: ATCCCAACGTCATCCAGCTC | 2 | 193-194 |
| R: CAGCAGGAAGGTGTGCAG | ||||||
| Pprm6 | XV | 8323413 | (CA)13 | F: CTGGAGCGTTTACAGGTGG | 8 | 364-382 |
| R: CTGCTGAGCTGAACAGGC | ||||||
| Pprm7 | XVI | 3587216 | (AC)10 | F: CTGGAGACCAACAAGTTGAGG | 12 | 274-298 |
| R: CTTAACAAAGATCCTGCTGGACG | ||||||
| Pprm8 | XVIII | 10203501 | (GT)25 | F: CACCCATGTTCCTGTGCTTC | 11 | 306-380 |
| R: ACAAAGCCCTGCTCTCGAG | ||||||
| Pprm9 | XX | 5366535 | (CA)14 | F: TCCTCATGATGTTGACCAGTGC | 3 | 166-172 |
| R: CTGGCCTATGGAAACCAGG | ||||||
LG, linkage group. F, forward; R, reverse. A, number of observed alleles.