Table 2. Functional enrichment among significantly differentially expressed genes between original cells and subclones.
| Original cell | Subclone cell | No. clusters | No. genes | Top 5 clusters | ||
| ES>2 | ES>2 | ES | No. genes | Annotations | ||
| Huh-7 | Huh-7.5.1 | 15 | 866 | 5.81 | 565 | Extracellular and secreted; signal peptide; glycoprotein; disulfide bond. |
| 4.93 | 119 | Response to hormone stimulus and organic substance. | ||||
| 3.92 | 47 | Response to steroid hormone and glucocorticoid stimulus. | ||||
| 3.52 | 48 | Response to extracellular stimulus, nutrients, retinoic acid and vitamin A. | ||||
| 3.37 | 37 | Complement and coagulation cascades; acute inflammatory and defense response. | ||||
| Huh-7.5.1 | Huh-7.5.1c2 | 2 | 108 | 7.09 | 83 | Glycoprotein; signal peptide; secreted; disulfide bond. |
| 4.93 | 74 | Response to hormone stimulus and organic substance. | ||||
| 10.55 | 416 | Extracellular and secreted; signal peptide; glycoprotein; disulfide bond. | ||||
| Huh-7.5.1 | R1.1 | 18 | 704 | 5.03 | 88 | Response to wounding; acute inflammatory and defense response. |
| 4.42 | 113 | Mitosis; organelle fission; cell-cycle; M-phase. | ||||
| 4.23 | 54 | Enzyme inhibitor; endopeptidase and protease inhibitor; SERPIN family; reactive bond. | ||||
| 3.60 | 50 | Proteinaceous extracellular matrix; basement membrane. | ||||
| 11.32 | 108 | DNA replication and DNA metabolic process. | ||||
| R1.1 | R1.09 | 13 | 582 | 7.07 | 151 | Mitosis; organelle fission; cell division; chromosome segregation;cell-cycle; M-phase. |
| 6.41 | 96 | Chromosomal part; centromeric region; chromatin. | ||||
| 5.46 | 140 | DNA repair; response to DNA damage; stress response. | ||||
| 4.85 | 45 | Condensed chromosome; kinetochore; centromeric region . | ||||
| R1.1 | R1.10 | 2 | 108 | 4.12 | 55 | Extracellular space. |
| 4.09 | 108 | Secreted; signal peptide; glycoprotein; disulfide bond. | ||||
| 6.34 | 165 | Sequence-specific DNA binding; Homeobox DNA binding domain. | ||||
| Huh-7.5.1 | R2 | 25 | 1325 | 4.97 | 95 | Embryonic morphogenesis; appendage development. |
| 4.93 | 40 | Acute inflammatory response; acute phase. | ||||
| 4.74 | 153 | Acute inflammatory, wounding and defense response. | ||||
| 4.59 | 73 | Extracellular and secreted; signal peptide; glycoprotein; disulfide bond. | ||||
Shown are the number of functional annotation clusters that achieve an enrichment score (ES) of >2 and the number of differentially expressed genes that these clusters include. The right-most three columns give details of the top scoring annotation clusters (a maximum of 5 are shown).