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. 2011 Oct 25;6(10):e26766. doi: 10.1371/journal.pone.0026766

Figure 6. PIC induces apoptosis in primary cortical neurospheres.

Figure 6

Primary neurospheres were obtained from cerebral cortex cells isolated from WT and TLR3−/− embryos at GD14. Neurospheres were cultured for 7 days in the presence of EGF (20 ng/ml) and treated with PBS or PIC for 24 hours. (a, b) Representative histograms showing Annexin V-positive cells in PBS- or PIC-treated WT or TLR3−/− neurospheres. Numbers in gates represent the percentages of Annexin V+ cells. Three independent cell culture assays were performed with cells isolated from embryos (n = 12); data from one representative assay is shown. (c) Box plot showing the percentages of Annexin V+ cells in PBS- and PIC-treated neurospheres derived from WT and TLR3−/− embryos. Results are expressed as percentages of total cells (*, p<0.05 relative to PBS control group; Mann-Whitney U test). Height of box plot shows inter-quartile range; horizontal line, median. Due to the low numbers involved in the flow cytometry experiments, the box plot upper and lower interquartile ranges also represent the maximum and minimum scores. White bars: PBS-treated mice, hatched bars: PIC-treated mice.