Table 2. Image intensities (Arbitrary Units) showing the fluorescence intensities in cardiac fibroblasts upon staining with DCF-DA, DHE, DAF-2DA and MitoTracker Red.
S. No. | Staining | Control | Tβ4 | H2O2 | H2O2 + Tβ4 |
1 | DCF-DA (H2O2) | 259± 48 | 96±19* | 614±26* | 183±41# |
2 | DHE (O2 - radicals) | 428±52 | 461±81 | 892±62* | 659±35# |
3 | DAF-2DA (NO) | 305±43 | 274±43 | 948±47* | 308±41# |
4 | MitoTracker Red (Δψm) | 644±58 | 657±73 | 253±45* | 618±38# |
Data acquired from at least 15 fields taken from 3–4 different confocal images of the same treatment group and were quantified by using ImageJ Software.
*denotes p<0.05 compared to controls while.
denotes p<0.05, compared to the H2O2-treated group.