FIG. 1.
Effect of Reishi on cell viability. Cell viability was quantified from PI-stained, intact (nonapoptotic) nuclei or via cell titer assay (Promega). Cells in serum containing media were treated for 24 h with the indicated dose of whole mushroom Reishi extract. A: MCF10A (noncancerous mammary epithelial cells), and SUM-149. B: MDA-MB-468 and MDA-MB-435, A-172 or MiaPaCa carcinoma cell lines. C: SUM-149 IBC cells treated for 96 h with varying concentrations of Reishi extract. Mean from 4 independent experiments ± SEM. *P < 0.05, **P < 0.001 compared to control (without Reishi treatment).