FIG. 2.

Effect of Reishi on apoptosis. Apoptosis induction was studied with annexin V staining using the APOAC kit (Sigma). SUM-149 cells were seeded on sterile coverslips and were grown in 5% FBS media for 24 h prior to treatment with or without Reishi extract for an additional 24 h. A: Control cells show no presence of annexin V (red) and clear presence of 6-carboxyfluorescein (CFDA; green), while Reishi-treated cells show presence of both annexin V and 6-CFDA dyes, an indication of apoptosis. B: Columns, mean from 10 microscopic fields/coverslip from 4 independent experiments ± SEM. *P < 0.05 compared to control (without Reishi treatment).