Table 1.

Effect of inhibitors on plasmin-dependent C6 glioma cell-directed migration

Condition	% of control (Lam/P/t or Col/P/t)
Uncoated	1.0
Collagen I	1.0
Col/P/t	100.0
Col/P/t + anti-annA1	104.3
Col/P/t + anti-annA2	41.5
Lam	1.0
Lam/P/t	100.0
Lam/P/t + anti-annA1	134.7
Lam/P/t + anti-annA2	45.9
Lam/P/t + Leup, 150 μm	10.1
Lam/P/t + α2-PL, 500 nm	8.2
Lam/P/t + EACA, 10 mm	46.0
Lam/P/t + anti-tPA, 100 nm	42.0
Lam/P/t + Amil, 200 μm	70.0
Lam/P/t + Amil + anti-tPA	12.9
Lam/P/t + o-Phen, 200 μg/ml	90.0
Lam/P/t + TIMP1, 2 μg/ml	112.3
Lam/P/t + TIMP2, 2 μg/ml	108.6

C6 glioma cells were plated on laminin- or collagen I-coated porous filter inserts and incubated, as described, with or without plasminogen and tPA, and with or without monoclonal anti-annexin A2, anti-annexin A1 IgG (50 μg/ml), or other inhibitors. n = 3–4. Col, Collagen; P, plasminogen; t, tPA; Lam, laminin; Leup, leupeptin; α2-PL, α2 anti-plasmin; EACA, ϵ-aminocaproic acid; Amil, amiloride; o-Phen, o-phenanthroline.