Figure 3. Absence of primary cilia increases endothelial monolayer permeability.

Serum starved confluent monolayers of wild type (WT), Tg737 (Tg737^orpk/orpk) and pkd1 null (pkd1^null/null) endothelial cells in 24 well fluoroblock tranwell plates (BD Biosciences) were incubated with 1ug/ml Alexa Fluor-488- dextran (3 kDa). The transfer of dextran into the bottom chamber was measured overtime in the presence or absence of thrombin (1U/ml). The graph represents the maximal change (at 11 min) in the fluorescence. The results shown are mean ± SEM from 3–4 independent experiments.