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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Gastroenterology. 2011 Jul 7;141(5):1738–1748.e2. doi: 10.1053/j.gastro.2011.06.070

Figure 2. DARPP-32 blocks gefitinib-induced apoptosis in gastric cancer cells.

Figure 2

A) The MKN-28 cells stably expressing DARPP-32 (DP01 and DP02) or empty vector were analyzed using the Annexin V-FITC and propidium iodide (PI) following the treatment with gefitinib (25 μM) or vehicle overnight. The early apoptotic cells, typically Annexin V positive and PI negative were indicated in the bottom right quadrant. Summary of the data is shown on the right panel. B) Western blot analyses of β-Actin, PARP, caspase 9 and caspase 3 proteins. C) The results in (A) and (B) were validated using the SNU-16 cells following knockdown of DARPP-32 with lentiviral particles (10 MOI) expressing DARPP-32 shRNA or control shRNA.