Table 2.
Summary of the effect of compounds activating or inhibiting cyclic nucleotide signaling on cell migration of VILIP-1-negative SCC CC4A, CH72T3 and VILIP-1-positive SCCs CC4B, CH72 in a wound closing assay.
| VILIP-1-negative SCC | ||||
|---|---|---|---|---|
| reagent | CC4A | CH72T3 | ||
| effect in % of ctr | p-value (n,N) | effect in % of ctr | p-value (n,N) | |
| 8Br-cAMP | −52% | < 0,0001 (10,3) | −51% | < 0,0001 (10,3) |
| FSK | −67% | < 0,0001 (10,3) | −40% | < 0,0001 (10,3) |
| 8Br-cGMP | +40 % | < 0,0001 (10,3) | +36% | < 0,0001 (10,3) |
| ANP | +21% | 0,0085 (10,3) | + 48% | < 0,0001 (10,3) |
| Epac-activator (8CPT-2Me-cAMP) | −19 % | 0,017 (5,2) | −23% | 0,0108 (5,2) |
| 8Br-cAMP and 8Br-cGMP | −44% (+17% compared to 8Br-cAMP) |
0,012 (5,2) (0,1539) |
−41% (+22% compared to 8Br-cAMP) |
< 0,0001 (5,2) (0,022) |
| VILIP-1-positive SCC | ||||
| reagent | CC4B | CH72 | ||
| effect in % of ctr | p-value (n,N) | effect in % of ctr | p-value (n,N) | |
| H89 | +17% +21% |
0,007 (8,3) 0,0069 (12,3) |
+68% +55% |
0,003 (8,3) 0,0003 (12,3) |
| DDA | +44% | 0,0042 (6,2) (0,074 vs H89) |
+63% | 0,0002 (6,2) (0,5368 vs H89) |
| KT5823 | −13% | 0,4949 (8,3) | −12% | 0,352 (8,3) |