Table 3.

In vivo toxicity of different active components of plants products against the cercaria larva of Fasciola gigantica.

Exposure	Larvicidal (mg/L)	LC50	LCL	UCL	Slope value	t-ratio	g-value	Heterogeneity
2 h	Citral	49.03	34.81	112.16	1.30 ± 0.34	3.80	0.26	0.14
	Ferulic acid	2.53	0.13	4.19	1.57 ± 0.35	4.50	0.19	0.20
	Umbelliferone	3.76	2.71	7.62	1.17 ± 0.33	3.56	0.30	0.17
	Azadirachtin	0.43	0.31	0.90	1.29 ± 0.33	3.81	0.26	0.13
	Allicin	2.80	1.75	7.00	0.79 ± 0.23	3.32	0.34	0.13
4 h	Citral	24.33	18.14	33.16	1.40 ± 0.33	4.24	0.21	0.13
	Ferulic acid	1.65	1.26	2.31	1.43 ± 0.33	4.23	0.21	0.17
	Umbelliferone	1.64	0.87	2.28	1.14 ± 0.32	3.50	0.31	0.14
	Azadirachtin	0.23	0.16	0.32	1.22 ± 0.32	3.75	0.27	0.11
	Allicin	1.06	0.50	1.60	0.92 ± 0.24	3.82	0.26	0.16
6 h	Citral	14.63	9.92	18.53	1.70 ± 0.34	4.46	0.15	0.12
	Ferulic acid	0.98	0.65	1.26	1.53 ± 0.34	4.46	0.19	0.18
	Umbelliferone	1.09	0.45	1.53	1.34 ± 0.33	3.96	0.24	0.09
	Azadirachtin	0.14	0.07	0.19	1.26 ± 0.33	3.82	0.26	0.12
	Allicin	0.42	0.10	0.74	1.02 ± 0.26	3.87	0.25	0.29
8 h	Citral	8.65	3.24	12.73	1.37 ± 0.34	3.94	024	0.19
	Ferulic acid	0.59	0.27	0.82	1.54 ± 0.36	4.21	0.21	0.21
	Umbelliferone	0.92	0.61	1.16	1.34 ± 0.33	5.78	0.11	0.30
	Azadirachtin	0.09	0.05	0.12	1.94 ± 0.37	5.13	0.14	0.39
	Allicin	0.38	0.16	0.58	1.65 ± 0.34	4.84	0.16	0.44

LCL: lower confidence limits, UCL: upper confidence limits. Citral, ferulic acid, umbelliferone, neem oil, azadirachtin, and allicin. Six batches of 15 snails were exposed to different concentration of the above molluscicidal treatments. Mortality of cercaria was recorded every 2 h. Concentrations given are the final concentration (W/V) in the glass aquarium water. Significant negative regression (P < 0.05) was observed between exposure time and LC₅₀ of treatments. TS: testing significant of the regression coefficient. Citral: 11.02⁺⁺, ferulic acid: 8.35⁺, umbelliferone: 1.56⁺⁺, azadirachtin: 11.82⁺⁺, allicin: 8.52⁺⁺: linear regression between x and y, ⁺⁺: nonlinear regression.