Figure 1.

Separation of apoplastic proteins by native-PAGE. Apoplastic proteins were extracted from cold-acclimated (CA) and nonacclimated (NA) rye leaves by vacuum infiltration followed by centrifugation. The apoplastic proteins were separated from equal volumes of unconcentrated apoplastic extracts (lanes CA and NA, 1) and from equal amounts (10 μg) of apoplastic proteins (lanes CA and NA, 2) in an 8% continuous native-polyacrylamide gel. The gel was stained with Coomassie brilliant blue R-250. Numbers on the left refer to individual native apoplastic proteins.