Figure 2.

Examination of polypeptides in cold-acclimated and nonacclimated apoplastic extracts by SDS-PAGE and immunoblotting. A, SDS-PAGE (15% acrylamide) of equal volumes of crude cold-acclimated and nonacclimated apoplastic extracts (lanes 1 and 2, respectively) and equal amounts (5 μg) of cold-acclimated and nonacclimated apoplastic proteins (lanes 1 and 3, respectively). Polypeptides were stained with Coomassie brilliant blue R-250. Low-range prestained SDS-PAGE molecular-mass standards from Bio-Rad are shown on the left (lanes M). SDS-PAGE gels with the same sample-loading scheme shown in A were blotted and probed with anti-GLP antiserum (B), anti-CLP antiserum (C), and anti-TLP antiserum (D). Positive immunodetection of polypeptides is indicated by arrows on the right.