Figure 4.

Specificities of anti-GLP, anti-CLP, and anti-TLP antisera. CA, Cold-acclimated apoplastic proteins (5 μg) were denatured, separated by 15% SDS-PAGE, and stained with Coomassie brilliant blue R-250. Similar gels were blotted and probed with antisera at a dilution of 1:3. Lane GLP was probed with anti-GLP antiserum, lane CLP was probed with anti-CLP antiserum, and lane TLP was probed with anti-TLP antiserum. Low-range prestained SDS-PAGE molecular-mass standards from Bio-Rad (lane M) were used to determine the molecular masses (kD). The molecular mass of each polypeptide immunodetected by an antiserum is indicated on the right.