Figure 6.

Immunoprecipitation of native AFPs by antisera produced against specific AFPs. Equal amounts (5 μg per lane) of proteins immunoprecipitated by anti-GLP (lanes 1), anti-CLP (lanes 2), or anti-TLP (lanes 3) antiserum, or by preimmune serum (lanes 4), were denatured and separated by 15% SDS-PAGE. Cold-acclimated apoplastic extract is shown as a positive control in lanes 5. A, Gel stained with Coomassie brilliant blue R-250. Gels were blotted and probed with anti-GLP antiserum (B), anti-CLP antiserum (C), and anti-TLP antiserum (D). Low-range prestained SDS-PAGE molecular-mass standards from Bio-Rad (lanes M) were used to determine the molecular masses (kD).