Figure 7.

Purification and identification of NP3. Cold-acclimated (CA) rye apoplastic extract and purified NP3 were examined by native-PAGE (A), SDS-PAGE (B), and immunoblotting (C). NP3 was purified from rye apoplastic extract by chitin-affinity chromatography. Low-range prestained SDS-PAGE molecular-mass standards from Bio-Rad (lanes M) were used to determine the molecular masses (kD). The gels in A and B were stained with Coomassie brilliant blue R-250. Anti-GLP and anti-CLP antisera were used to detect GLP and CLP, respectively.