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Antibody specificity against PLDα, PLDβ, and PLDγ. Purified GST-PLDα, GST-PLDβ, and GST-PLDγ (0.1 μg per lane) were separated by 8% SDS-PAGE. The blots were incubated with anti-PLDα, anti-PLDβ, or anti-PLDγ polyclonal antibodies (1:1000 dilution) and then the second antibody. PLD-antibody complexes were made visible by alkaline phosphatase.
