Fig. 3.

Treg suppressive function was not influenced by effector T cells. CD4⁺CD25⁻ T cells from a PCa patient pre-vaccination (a) and post-vaccination (b) were cultured alone or at a 1:1 ratio with Tregs isolated from the same PCa patient post-vaccination, upon stimulation with anti-CD3 and irradiated T cell-depleted PBMCs. CD4⁺CD25⁻ T cells from a PCa patient pre-vaccination (c) and post-vaccination (d) were cultured alone or at a 1:1 ratio with Tregs isolated from the same PCa patient pre-vaccination, upon stimulation with anti-CD3 and irradiated T cell-depleted PBMCs. Experiments were performed in triplicate. Columns means (cpm), bars SD. Percentages indicate level of inhibition