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Genetic structure of PAI II536. For the transfer experiments, suicide vector pSG704 which carries the chloramphenicol acetyltransferase (cat) gene, an origin of replication and mobility genes (depicted in the enlarged insert) was stably integrated into a non-coding region of this island (A). Complete transfer of PAI II536 into the transconjugants was confirmed by detection of five regions of PAI II536 by PCR (B).
