Table 2.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| E. coli strains | ||
| 536 wt | UPEC wild type strain, SmR | [69] |
| 536-21 | 536, ΔPAI I536 ΔPAI II536, SmR | [2] |
| 536-19/1mob | Donor strain in the mobilisation experiments, pirλatt, mobGP704 inserted in PAI II536, pRP4, SmR, ApR, CmR, TcR, KmR | This study |
| SY327λpir | F-, araD, Δ(lac pro), argE(Am), recA56, RifR, gyrA λpir | [60] |
| SM10λpir | thi1, thr1, leuB6, supE44, tonA21, lacY1, recA::RP4-2-Tc::Mu λpir KmR | [60] |
| SY327-23 | Mobilised PAI II536 is integrated into leuX | This study |
| SY327-77 | Mobilised PAI II536 is present as a CI | This study |
| Plasmids | ||
| pGEM®T-Easy | bla, T/A cloning vector | Promega |
| pGP704 | bla, oriR6K, mobRP4 | [60] |
| pSG704 | cat, oriR6K, mobRP4 | This study |
| pCVD442Tc | bla, tet, oriR6K, mobRP4, sacB | This study |
| pLDR8 neo, int expression vector, Ts | neo, int expression vector, Ts | [62] |
| pLDR9 | bla neo, cloning vector to integrate DNA into attB | [62] |
| pPAI II-CI | bla, positive control for detection of PAI II536-specific CIs | [17] |