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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Bipolar Disord. 2011 Aug;13(5-6):474–485. doi: 10.1111/j.1399-5618.2011.00941.x

Fig. 3.

Fig. 3

SP4 protein stability is regulated by depolarization. Cerebellar granule neurons were obtained from P6 rat pups and maintained in culture for 7 days in vitro (DIV-7). (A) Neurons were incubated in medium with 25 mM or 5 mM KCl in the presence of serum for the indicated times. Protein extracts were probed with antisera against SP4 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (B) Granule neurons were incubated in medium with 25 mM or 5 mM KCl in the presence of serum for one hour. Protein extracts were resolved by SDS/electrophoresis and probed with a polyclonal antibody against Sp proteins or GAPDH as indicated. Sp protein levels were analyzed by densitometry and normalized to GAPDH levels. Values represent mean ± standard deviation. (C) At DIV-7, cerebellar granule neurons were incubated with 30 μM nimodipine or the vehicle (DMSO) for 12 hours. SP4 protein levels were analyzed by densitometry and normalized to GAPDH levels. Values represent mean ± standard deviation of three independent experiments. Statistical analysis was performed using two-tailed t-test (*p < 0.05; **p < 0.01).