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. 2011 Jul 4;99(1):211–225. doi: 10.1093/bmb/ldr025

Fig. 1.

Fig. 1

Tensile loading plays a crucial role in tenocytes. (a) Achilles tendons in adult Scleraxis-GFP transgenic mice. Left panel: under fluorescence stereomicroscope; right panel: under microscope with GFP/ultraviolet (UV) filters to show scleraxis-GFP (green) and nucleus [4′6-diamidino-2-phenylinodole (DAPI) blue]. AT, Achilles tendon. Bar = 100 µm. (b) Analysis of cell death at 2 h after complete transection of adult Achilles tendon in Scleraxis-GFP transgenic mice. Arrows indicate the transection edge of tendons. Note that the expression of scleraxis-GFP (green) is diminished and cells positive by terminal deoxynucleotidyl transferase dUTP nick-end labeling assay (TUNEL assay: a marker for cell death, red) are evident in the transected Achilles tendon. Bar = 100 µm.