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. Author manuscript; available in PMC: 2011 Oct 26.
Published in final edited form as: J Chromatogr A. 2008 Feb 20;1192(1):54–61. doi: 10.1016/j.chroma.2008.02.049

Fig. 1.

Fig. 1

Importance of the flush-out volume on the separation of tryptic peptides of bovine serum albumin (BSA). Conditions: SB-C18 (150 × 4.6 mm; 5 mm; 80 Å; Vm ~ 1.6 mL ); Eluent A: 5/94.9/0.1 ACN/H2O/TFA; Eluent B: 55/44.9/0.1 ACN/H2O/TFA; 0/100 to 20/80 A/B in 12 min; hold for 1 min; 2 mL/min; HP 1100 (VD ~ 0.90 mL; Vflush ~ 2.5 mL) ; 40 oC; 15 µL injection of tryptic peptides of BSA prepared as described in ref. [7]; 220 nm detection. The reequilibration time (tre) is the time between the end and start of two consecutive gradient runs.