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. Author manuscript; available in PMC: 2011 Oct 26.
Published in final edited form as: J Chromatogr A. 2008 Feb 20;1192(1):54–61. doi: 10.1016/j.chroma.2008.02.049

Fig. 2.

Fig. 2

Plot of the difference in the retention time (tR) from the control and experimental re-equilibration times as a function of the reequilibration volume. The inner plot is a “blow-up” of the larger plot. Conditions: 15 cm × 4.6 mm I.D. column packed with 5 mm SB-C18 80 Å particles; eluent A: 1/10/88.9/0.1 BuOH/MeCN/H2O/TFA; eluent B: 1/90/8.9/0.1 BuOH/MeCN/H2O/TFA; 100/0 to 0/100 to 0/100 A/B in 10 mL to 11 mL at 2 mL/min; VD = 0.90 mL; Vflush ~ 2.5 mL; 40 °C. The solutes in the red rectangle elute within the “critical region” after the dead time (at around 0.72 minutes) between 0.8 and 1.3 minutes. The dashed horizontal lines represent the range in time where the retention of the solute is similar to the repeatability and thus considered to be statistically constant. The “worst” solute under these and other conditions was always pheniramine unless otherwise noted.