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. Author manuscript; available in PMC: 2011 Oct 26.
Published in final edited form as: Methods Mol Biol. 2010;610:165–180. doi: 10.1007/978-1-60327-029-8_10

Table 1.

A: Inhibitory effect of eugenol on Fe(II)-ascorbate induced lipid peroxidation
in rat liver mitochondria.
Antioxidant Mitochondri
(ml)
KCl-
Tris
Buffer
(ml)
70%
ethanol
Antioxidant Final
antioxidant
concentration
Ascorbate
(200μM)
Fe(II)
(10μM)
OD at
530
MDA
(nmol/
mg
protein)
0 0.5 1.5 - - - - - 0.011 0.705
0 (cont) 0.5 1.46 10μl - - 20 μl 10 μl 0.673 31.7
Eugenol 2 mM 0.5 1.46 ---- 10 μl 10 μM 20 μl 10 μl 0.350 16.5
Eugenol 4 mM 0.5 1.46 ---- 10 μl 20 μM 20 μl 10 μl 0.154 7.24
Eugenol 6 mM 0.5 1.46 ---- 10 μl 30 μM 20 μl 10 μl 0.078 3.67
Eugenol 8 mM 0.5 1.46 ---- 10 μl 40 μM 20 μl 10 μl 0.043 2.02
Eugenol 10 mM 0.5 1.46 ---- 10 μl 50 μM 20 μl 10 μl 0.009 0.476
α-tocoph 10 mM 0.5 1.46 -- 10 μl 50 μM 20 μl 10 μl 0.375 17.7
BHT 0.25 mM 0.5 1.46 -- 10 μl 1.25 μM 20 μl 10 μl 0.324 15.25
B. MDA standards
MDA (nmoles) MDA Std
volume
Buffer OD at
532
0.0 0.0 2 ml 0.008
0.625 31.25 1.969 0.034
1.25 62.5 1.937 0.059
2.5 125 1.875 0.133
5.0 250 1.750 0.222
10.0 500 1.5 0.438