Table 1.
A: Inhibitory effect of eugenol on Fe(II)-ascorbate induced lipid peroxidation in rat liver mitochondria. | |||||||||
---|---|---|---|---|---|---|---|---|---|
Antioxidant | Mitochondri (ml) |
KCl- Tris Buffer (ml) |
70% ethanol |
Antioxidant | Final antioxidant concentration |
Ascorbate (200μM) |
Fe(II) (10μM) |
OD at 530 |
MDA (nmol/ mg protein) |
0 | 0.5 | 1.5 | - | - | - | - | - | 0.011 | 0.705 |
0 (cont) | 0.5 | 1.46 | 10μl | - | - | 20 μl | 10 μl | 0.673 | 31.7 |
Eugenol 2 mM | 0.5 | 1.46 | ---- | 10 μl | 10 μM | 20 μl | 10 μl | 0.350 | 16.5 |
Eugenol 4 mM | 0.5 | 1.46 | ---- | 10 μl | 20 μM | 20 μl | 10 μl | 0.154 | 7.24 |
Eugenol 6 mM | 0.5 | 1.46 | ---- | 10 μl | 30 μM | 20 μl | 10 μl | 0.078 | 3.67 |
Eugenol 8 mM | 0.5 | 1.46 | ---- | 10 μl | 40 μM | 20 μl | 10 μl | 0.043 | 2.02 |
Eugenol 10 mM | 0.5 | 1.46 | ---- | 10 μl | 50 μM | 20 μl | 10 μl | 0.009 | 0.476 |
α-tocoph 10 mM | 0.5 | 1.46 | -- | 10 μl | 50 μM | 20 μl | 10 μl | 0.375 | 17.7 |
BHT 0.25 mM | 0.5 | 1.46 | -- | 10 μl | 1.25 μM | 20 μl | 10 μl | 0.324 | 15.25 |
B. MDA standards | |||
---|---|---|---|
MDA (nmoles) | MDA Std volume |
Buffer | OD at 532 |
0.0 | 0.0 | 2 ml | 0.008 |
0.625 | 31.25 | 1.969 | 0.034 |
1.25 | 62.5 | 1.937 | 0.059 |
2.5 | 125 | 1.875 | 0.133 |
5.0 | 250 | 1.750 | 0.222 |
10.0 | 500 | 1.5 | 0.438 |