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. Author manuscript; available in PMC: 2011 Oct 26.
Published in final edited form as: Methods Mol Biol. 2010;610:165–180. doi: 10.1007/978-1-60327-029-8_10

Table 3.

Effect of eugenol treatment on serum GOT, microsomal P450, G-6-Pase and hepatic necrosis in rats administered CCl4.

Treatment Serum GOT U/ml Cyt- P450 nmol/mg protein G-6-Pase μmol Pi released /hr/mg protein Hepatic necrosis score
Control 18 ± 4 0.74 ± 0.10 10.5 ± 0.57 0.0
CCl4 (0.4ml/kg) 380 ± 45 0.38 ± 0.04* 2.6 ±0.41* 2.0 ± 0.0
CCl4 (0.4ml/kg) + Eugenol (0.2mg/kg) 225 ± 35* 0.36 ± 0.03* 2.8 ± 0.24* 2.0 ± 0.0
CCl4 (0.4ml/kg) + Eugenol (1.0 mg/kg) 160 ± 20** 0.41 ± 0.04* 2.4 ± 0.11* 1.5 ± 0.28*
CCl4 (0.4ml/kg) + Eugenol (5.0 mg/kg) 150 ± 21*** 0.34 ± 0.028* 2.5 ± 0.22* 1.25 ± 0.25*
CCl4 (0.4ml/kg) + Eugenol (25mg/kg) 220 ± 27* 0.45 ± 0.036* 2.2 ± 0.10* 2.0 ± 0.25

Serum is collected after 24 hrs of CCl4 and assayed for GOT activity. An asterisk indicated a significant difference between CCl4 and eugenol + CCl4 treatment.

*

p< 0.01,

**

P<0.01,

***

P<0.01.

Rats are sacrificed after 48 hrs of CCl4 dose. Microsomal cyto-p450, G-6-phosphatase* significantly different from controls, P< 0.01 and liver necrosis, * significantly different from CCl4 treatment, P< 0.01.

Results are mean ± SE for 4-6 rats