Table 3.
Treatment | Serum GOT U/ml | Cyt- P450 nmol/mg protein | G-6-Pase μmol Pi released /hr/mg protein | Hepatic necrosis score |
---|---|---|---|---|
Control | 18 ± 4 | 0.74 ± 0.10 | 10.5 ± 0.57 | 0.0 |
CCl4 (0.4ml/kg) | 380 ± 45 | 0.38 ± 0.04* | 2.6 ±0.41* | 2.0 ± 0.0 |
CCl4 (0.4ml/kg) + Eugenol (0.2mg/kg) | 225 ± 35* | 0.36 ± 0.03* | 2.8 ± 0.24* | 2.0 ± 0.0 |
CCl4 (0.4ml/kg) + Eugenol (1.0 mg/kg) | 160 ± 20** | 0.41 ± 0.04* | 2.4 ± 0.11* | 1.5 ± 0.28* |
CCl4 (0.4ml/kg) + Eugenol (5.0 mg/kg) | 150 ± 21*** | 0.34 ± 0.028* | 2.5 ± 0.22* | 1.25 ± 0.25* |
CCl4 (0.4ml/kg) + Eugenol (25mg/kg) | 220 ± 27* | 0.45 ± 0.036* | 2.2 ± 0.10* | 2.0 ± 0.25 |
Serum is collected after 24 hrs of CCl4 and assayed for GOT activity. An asterisk indicated a significant difference between CCl4 and eugenol + CCl4 treatment.
p< 0.01,
P<0.01,
P<0.01.
Rats are sacrificed after 48 hrs of CCl4 dose. Microsomal cyto-p450, G-6-phosphatase* significantly different from controls, P< 0.01 and liver necrosis, * significantly different from CCl4 treatment, P< 0.01.
Results are mean ± SE for 4-6 rats