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. Author manuscript; available in PMC: 2011 Oct 26.
Published in final edited form as: Virology. 2010 May 6;403(1):92–102. doi: 10.1016/j.virol.2010.03.052

Figure 1. KSHV-permissive cells express extracellular Hsp90.

Figure 1

(A–D) To identify cell surface localization of Hsp90 (csHsp90) by flow cytometry, HeLa (A–C) and primary dermal microvascular endothelial cells (pDMVEC) (D) were incubated with antibodies recognizing either the C-terminus (A), N-terminus (B and D), or an uncharacterized epitope (C) of the alpha isoform of Hsp90 followed by secondary antibodies conjugated to ALEXA-488 (black histograms). For controls, cells were incubated with secondary antibodies alone (gray histograms). (E–G) To validate these results, HeLa cells incubated with secondary antibodies conjugated to ALEXA-488 (E), anti-C-terminal Hsp90 Ab and secondary antibodies (F), or anti-N-terminal Hsp90 Ab and secondary antibodies (G) were examined by fluorescence microscopy. Original magnification × 60. Data shown represent one of three independent experiments.